ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (2): 317-322.doi: 10.11843/j.issn.0366-6964.2015.02.020

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Effect of the Antagonist for the Combined Anaesthetic for Miniature Pigs on p-p38 Protein and c-myc Gene in Brain Regions of Rats

LI Yan#,HOU Jin-long#,NIU Dong-liang,JIANG Sheng,SHI Jing,FAN Hong-gang,WANG Hong-bin*   

  1. (College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China)
  • Received:2014-04-10 Online:2015-02-23 Published:2015-02-23

Abstract:

In order to investigate the effects of antagonist for the Combined Anaesthetic for Miniature Pigs (XFM) on p-p38 protein and c-myc gene in different encephalic region of rats,and discuss the mechanism of XFM antagonist.Eighteen Wistar rats were divided randomly into C (Control group) and J (Antagonist group) group.J group consisted of two subgroups,J1(5 min after rats received the XFM antagonist intraperitoneally) and J2 (1 h after rats received the XFM antagonist intraperitoneally).The brain tissues were collected at each time point.The expression of p-p38 in different brains regions were detected by Western blot.The transcription of c-myc mRNA in different brains regions were detected by real time PCR.The results showed that the expression of p-p38 and c-myc mRNA in cerebral cortex,thalamus and brain stem increased significantly;the expression of p-p38 and c-myc mRNA in cerebellum and hippocampus decreased significantly.These results indicated that p-p38 protein and c-myc gene were affected by the XFM antagonist,this may be one of the antagonism mechanisms.

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